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制备脂质体包裹的Ag85A口服DNA疫苗,并观察小鼠口服后所诱生的抗体产生情况。用脂质体包裹重组质粒pcDNA3.1/myc-HisA-Ag85A制备口服DNA疫苗,并用脂质体包裹空质粒pcDNA3.1/myc-HisA作为对照。将C57BL/6小鼠随机分为3组,即生理盐水组、空质粒组和重组质粒DNA疫苗组。分别将生理盐水、空质粒和重组质粒DNA疫苗以灌胃方式投给各组小鼠,共免疫3次,每次间隔14d,末次免疫后14d处死小鼠,ELISA法检测血清中Ag85A特异性抗体水平,放射免疫法测定肠组织中分泌型IgA(sIgA)含量。重组质粒组血清中Ag85A特异性抗体滴度为1160,空质粒组和生理盐水组血清中均未捡出Ag85A特异性抗体。重组质粒组肠组织中sIgA含量(0.3761±0.0456)μg/mL较空质粒组(0.2374±0.0414)μg/mL和生理盐水(0.1993±0.0899)μg/mL组显著增高(P<0.05),而空质粒组和生理盐水组未见有意义的变化(P>0.05)。口服脂质体包裹Ag85ADNA疫苗可诱导外周特异性抗体的产生和肠道黏膜局部sIgA的水平的升高。
Preparation of liposome-encapsulated Ag85A oral DNA vaccine, and observed the mice induced by oral antibody production. The recombinant plasmid pcDNA3.1 / myc-HisA-Ag85A was packaged by liposome to prepare oral DNA vaccine, and the empty plasmid pcDNA3.1 / myc-HisA was used as a control. C57BL / 6 mice were randomly divided into three groups: saline group, empty plasmid group and recombinant plasmid DNA vaccine group. Saline, empty plasmid and recombinant plasmid DNA vaccine were intragastrically administered to each group of mice, a total of three immunizations, each interval 14d, 14d after the last immunization of mice were killed, ELISA detection of serum Ag85A specific antibodies The levels of secretory IgA (sIgA) in intestinal tissue were measured by radioimmunoassay. Ag85A-specific antibody titer in the recombinant plasmids serum was 1160, Ag85A-specific antibodies were not detected in the serum of the empty plasmid group and the saline group. The sIgA content of the intestine of the recombinant plasmid group was significantly higher (0.3761 ± 0.0456 μg / mL vs 0.2374 ± 0.0414 μg / mL vs 0.1993 ± 0.0899 μg / mL than the control group (0.1993 ± 0.0899 μg / mL, There was no significant change in plasmid group and saline group (P> 0.05). Oral liposome-encapsulated Ag85ADNA vaccine induced the production of peripheral-specific antibodies and increased levels of intestinal sIgA in the intestinal mucosa.