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本实验对人类白细胞抗原（ＨＬＡ）、爱波斯坦－巴尔病毒核抗原（ＥＢＮＡ）、Ｔ细胞亚群的表达及分布进行综合分析，探讨其在癌变过程中的作用。用单克隆抗体Ｗ＿６／３２（抗ＨＬＡ－Ａ，Ｂ，Ｃ）、ＣＲ＿３／４３（抗ＨＬＡ－ＤＲ，ＤＰ，ＤＱ）、和Ｔ＿４、Ｔ＿８、Ｔ＿（１１），以碱性磷酸酶抗碱性磷酸酶法（ＡＰＡＡＰ）和用鼻咽癌患者血清以抗补体Ｃ＿３免疫荧光法，检测鼻咽癌（２５例）及慢性鼻咽炎（１０例）活检组织中ＨＬＡ、Ｔ细胞亚群分型和ＥＢＮＡ的表达。结果显示，鼻咽癌癌细胞多有ＨＬＡ－Ⅰ和Ⅱ型抗原表达，而以ＨＬＡ－Ⅱ型抗原表达为明显；慢性鼻咽炎上皮细胞表达ＨＬＡ－Ⅰ和Ⅱ型抗原较弱，与癌细胞比较，差异有显著性（Ｐ＜０．０５）。提示在癌变过程中，存在着ＨＬＡ表达的异常，体内ＥＢ病毒特异性Ｔ细胞可能不能识别带ＨＬＡ－Ⅱ型抗原的癌细胞，因而使其逃脱了机体的免疫监视。鼻咽癌组织中，癌细胞ＥＢＮＡ呈阳性和强阳性；癌间质中Ｔ细胞总数、Ｔ辅助、Ｔ杀伤细胞数量均减少，证明鼻咽癌局部细胞免疫功能降低。
In this study, the expression and distribution of human leukocyte antigen (HLA), Epstein-Barr virus nuclear antigen (EBNA) and T cell subsets were analyzed comprehensively to explore its role in carcinogenesis. The anti-alkaline phosphatase activity was measured using monoclonal antibodies W_6 / 32 (anti-HLA-A, B and C), CR_3 / 43 (anti-HLA- DR, DP and DQ), and T_4, T_8 and T_ (11) (APAAP) and nasopharyngeal carcinoma patients with anti-complement C_3 immunofluorescence method to detect HLA, T cell subsets and EBNA in nasopharyngeal carcinoma (25 cases) and chronic nasopharyngitis (10 cases) biopsies expression. The results showed that there were more HLA-Ⅰ and Ⅱ antigens in NPC cells and more obvious HLA-Ⅱ antigens. The expression of HLA-Ⅰ and Ⅱ antigens in chronic nasopharyngeal epithelial cells was weaker than that in cancer cells , The difference was significant (P <0.05). It is suggested that there is an abnormal expression of HLA during carcinogenesis. EB virus-specific T cells in vivo may not recognize cancer cells with HLA-Ⅱ type antigens, thereby avoiding the immune surveillance of the body. In nasopharyngeal carcinoma, EBNA of cancer cells was positive and strongly positive. The total number of T cells, T helper and T killer cells in cancer stroma decreased, which indicated that local cellular immune function of NPC was decreased.