论文部分内容阅读
目的 为了分离孕妇外周血中的有核红细胞用于非损伤性产前诊断。方法 采用了一种新的亲合素法 ,用红细胞表面半乳糖基特异的凝集素 (大豆凝集素 )来选择富集孕妇外周血中的有核红细胞。结果 从 11份 2 .5ml孕 14 + ～ 15 +w的孕妇外周血中都分离到了有核红细胞 ,平均每份血样得到有核红细胞 2 .8个。结论 本方法实验成本低 ,操作简单 ,对于非损伤性产前诊断的研究和推广都具有重要的参考价值。
Objective To isolate non-invasive prenatal diagnosis of nucleated erythrocytes in pregnant women’s peripheral blood. Methods A new avidin method was used to select enriched nucleated erythrocytes in peripheral blood of pregnant women by using hemagglutinin (Lectin) specific for galactosyl on erythrocyte surface. Results The nucleated erythrocytes were isolated from 11 2.5ml pregnant 14 + 15 + w pregnant women. On average, 2.8 nucleated erythrocytes were obtained from each blood sample. Conclusions This method has low experimental cost and simple operation. It has important reference value for the research and popularization of non-invasive prenatal diagnosis.