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目的：探讨形态学、免疫学和细胞遗传学（MIC）联合检测对急性早幼粒细胞白血病（M3）的临床意义。方法：将MIC技术用于35例M3患者的诊断、分型及预后评价。结果：形态学检查28例M3a中2例和7例M3v中3例曾误诊为其它白血病，经核型分析确诊。免疫分型10例，多为HLA-DR（－）、CD14（-）、CD33（＋）和CD13（＋）。骨髓染色体分析，正常3例（3／35），31例（31／35）有t（15；17），1例（1／35）具有变异易位．可评价的21例中19例（19／21）达到CR，具有复杂枝型和变异易位各1例未能取得缓解。结论：MIC联合检测在M3的诊断、分型、评价预后等有重要价值。
Objective: To explore the clinical significance of combined detection of morphology, immunology and cytogenetics (MIC) for acute promyelocytic leukemia (M3). Methods: MIC technique was used to diagnose, classify and evaluate the prognosis of 35 patients with M3. Results: Morphological examination of 28 cases of M3a and 2 cases of M3v were misdiagnosed as other leukemias. The diagnosis was confirmed by karyotype analysis. There were 10 cases of immunophenotyping, mostly HLA-DR(-), CD14(-), CD33(+) and CD13(+). Bone marrow chromosomal analysis was normal in 3 cases (3/35), 31 cases (31/35) had t(15; 17), and 1 case (1/35) had a mutated translocation. Of the 21 cases that could be evaluated, 19 (19/21) achieved CR, and one case with complicated branching and variant translocation failed to achieve remission. Conclusion: The combined detection of MIC has important value in the diagnosis, classification, and prognosis of M3.