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目的:观察溃疡性结肠炎相关癌变结肠黏膜β-catenin、C-myc表达变化,探讨β-catenin、C-myc在溃结癌变中的作用。方法:60只清洁级Balb/c小鼠随机分两组,每组30只,正常组、模型组;除外正常组,模型组采用DMH/DSS复合法制备溃结癌变模型,分别应用光镜检测结肠黏膜组织形态学变化,免疫组化及实时荧光定量PCR检测结肠黏膜β-catenin、C-myc蛋白及其mRNA表达变化。结果:模型组小鼠结肠肉眼可见浆膜层散在、多处息肉状隆起变化,镜下见腺体萎缩、高级别上皮内瘤变,部分癌变,其结肠β-catenin、C-myc蛋白及其mRNA呈高表达特点;与正常组比较,有统计学意义(P<0.05)。结论:β-catenin信号突变及下游靶基因C-myc过度转录在溃疡性结肠炎相关癌变发生发展中占据重要地位,调控β-catenin将有望成为防治溃疡性结肠炎相关癌变的靶点。
Objective: To observe the expression of β-catenin and C-myc in colorectal mucosa associated with ulcerative colitis and to explore the role of β-catenin and C-myc in canceration of ulcerated colorectal cancer. Methods: Sixty clean-grade Balb / c mice were randomly divided into two groups (30 rats in each group, normal group and model group). Except the normal group, DMU / DSS complex method was used to prepare the ulcerated colorectal cancer model. The morphological changes of colonic mucosa were observed. The expressions of β-catenin and C-myc in colonic mucosa were detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results: In the model group, there were scattered serosal layers in the colon and multiple polypoid protuberances. The glandular atrophy, high grade intraepithelial neoplasia, partial canceration, colon β-catenin, C-myc protein and their The mRNA expression was higher than that of the normal group (P <0.05). CONCLUSIONS: β-catenin signaling and C-myc overexpression of downstream target gene play an important role in the development of carcinogenesis associated with ulcerative colitis. The regulation of β-catenin may be the target of preventing and treating ulcerative colitis-related carcinogenesis.