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目的:观察足叶乙甙(VP16)与8-甲氧基补骨脂素(8-MOP)联合用药对人粘液表皮样癌高转移细胞株(Mc3)的抑制作用。方法:采用MTT法及克隆形成法观察VP16与8-MOP联合用药对粘液表皮样癌的抑制作用。结果:Mc3细胞对VP16有较高的敏感性,且呈显著的剂量依赖性,其IC50值为1625.67ng/ml;小剂量的8-MOP可刺激Mc3细胞增殖,高浓度的8-MOP则明显抑制Mc3细胞生长,其IC50为75500ng/ml;VP16与8-MOP联合应用时,采用分别低于VP16IC50的100、320和1000ng/ml药物浓度,联合用药的合并指数(CI)均小于0.95,分别为0.350、0.599和0.880;VP16对Mc3细胞的生长及克隆形成有明显的抑制作用,IC50为126ng/ml。结论:8-MOP与抗癌药物VP16联合应用,显示明显协同抑制效应,VP16对Mc3细胞克隆形成有抑制作用
OBJECTIVE: To observe the inhibitory effect of combined use of etoposide (VP16) and 8-methoxypsoralen (8-MOP) on human high-metastatic mucoepidermoid carcinoma cell line (Mc3). Methods: MTT assay and cloning method were used to observe the inhibitory effect of VP16 combined with 8-MOP on mucoepidermoid carcinoma. RESULTS: Mc3 cells were highly sensitive to VP16 in a dose-dependent manner with an IC50 value of 1625.67 ng/ml; low doses of 8-MOP stimulated proliferation of Mc3 cells, and high concentrations of 8-MOP were evident. Inhibition of Mc3 cell growth had an IC50 of 75,500 ng/ml; when VP16 was combined with 8-MOP, the drug concentrations of 100, 320, and 1000 ng/ml were lower than those of VP16IC50, respectively, and the combination indexes (CI) of the combined drugs were all less than 0.95, respectively. 0.350, 0.599, and 0.880; VP16 significantly inhibited the growth and colony formation of Mc3 cells with an IC50 of 126 ng/ml. Conclusion: The combination of 8-MOP and anticancer drug VP16 shows a clear synergistic inhibitory effect, and VP16 inhibits the formation of Mc3 cell clones.