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目的调查吉林省蜱粒细胞无形体感染。方法运用聚合酶链反应方法对吉林延边地区采集的蜱标本粒细胞无形体16S rRNA和gltA基因片段进行扩增及序列分析,将扩增序列与GenBank注册的基因序列进行比较,构建粒细胞无形体gltA基因进化树。结果共检测游离蜱427只,其中全沟硬蜱100只,森林革蜱327只,粒细胞无形体感染阳性率分别为4.00%和0.00%。寄生蜱感染阳性率2.9%。寄生蜱与游离蜱感染率差别无显著性。16S rRNA序列与我国已在GenBank注册的AF205140序列一致,与国外粒细胞无形体16S rRNA序列存在不同程度的差异,相似性为97%～99%;gltA基因与GenBank的粒细胞无形体gltA基因片段比较,相似性为87%～97%,推导的氨基酸序列相似性为84%～99%。结论我国吉林省林区存在蜱粒细胞无形体感染。
Objective To investigate the infection of tick granulocyte-like body in Jilin Province. Methods The 16S rRNA and gltA gene fragments were amplified and sequenced from the collected specimens of ticks in Yanbian, Jilin Province by polymerase chain reaction. The amplified sequences were compared with those registered in GenBank to construct an anaplastic granulocyte gltA gene phylogenetic tree Results A total of 427 ticks were detected. Among them, 100 ticks were ticked and 327 ticks were ticked. The positive rates of infection were 4.00% and 0.00% respectively. The positive rate of parasitic tick infection was 2.9%. There was no significant difference in the infection rates of parasitic ticks and free ticks. 16S rRNA sequence is consistent with the AF205140 sequence registered in GenBank. The 16S rRNA sequence has a similar degree of difference of 97% -99% with the foreign GG 16S rRNA sequence. The gltA gene is incompatible with the gltA gene fragment of GenBank The similarity was 87% -97%, and the deduced amino acid sequence similarity was 84% -99%. Conclusion There is a tick-like granulocyte-like body infection in Jilin Province.