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二乙醇胺基竹红菌乙素(2-ethanolamino-2-demethoxy-17-ethanolimino-hypocrellin B,EAHB)是一种新型的可吸收600 nm以上红光的竹红菌乙素衍生物。本文研究了二乙醇胺基竹红菌乙素-光动力诱导HeLa细胞死亡的效果及其氧化应激机制。结果发现,红光诱导后,MTT法检测到二乙醇胺基竹红菌乙素-光动力作用使HeLa细胞的存活率显著降低,且存活率与光敏剂浓度和光照剂量成反比;二乙醇胺基竹红菌乙素-光动力诱导HeLa细胞内产生活性氧自由基;同时,胞内超氧化物歧化酶和还原型谷胱甘肽水平显著降低,细胞脂质过氧化标志分子丙二醛显著升高,并检测到细胞质膜损伤标志分子乳酸脱氢酶的渗出显著增加。研究结果说明新型光敏剂二乙醇胺基竹红菌乙素可有效光诱导肿瘤细胞死亡,而细胞内氧化应激反应可能是二乙醇胺基竹红菌乙素光诱导肿瘤细胞死亡的重要作用机制。
2-ethanolamino-2-demethoxy-17-ethanolimino-hypocrellin B (EAHB) is a novel hypocrellin derivative that can absorb more than 600 nm red light. In this paper, the effect of diethanolamine-hypocrellin-B photodynamic therapy on HeLa cell death and its mechanism of oxidative stress were studied. The results showed that after induced by red light, the viability of HeLa cells was significantly reduced by MTT assay, and the survival rate was inversely proportional to the concentration of photosensitizer and the dosage of light. Diethanolamine-based bamboo Rhodotoridin-photodynamic therapy induced active oxygen free radicals in HeLa cells. At the same time, the levels of intracellular superoxide dismutase and reduced glutathione were significantly decreased, and malondialdehyde (MDA), a marker of lipid peroxidation, was significantly increased , And markedly increased the leakage of lactate dehydrogenase, a marker of plasma membrane damage. The results show that the new photosensitizer diethanolamine Hypocrellin B can effectively induce tumor cell death, and intracellular oxidative stress may be diethanolamine B hypocrellin light-induced tumor cell death an important mechanism of action.