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1989年Tepper等把白细胞介素4（IL-4）基因转入乳腺癌细胞发现可以诱导机体特异性抗肿瘤作用,从而拉开了基因瘤苗研究的序幕.白细胞介素2（IL-2）是抗原活化的TH细胞分泌的细胞因子,在兔疫功能调节,尤其是在抗肿瘤免疫调节中起着重要的作用.近年来,国内外学者对IL-2基因瘤苗的研制作了大量的工作,本文就此进展作一综述.1 基因转移技术把IL-2基因转染肿瘤细胞,并获得较高水平的表达,是转基因瘤苗研究的关键技术.理想的技术方法能够使目的基因高效地导入人肿瘤细胞,并且操作简便,具有安全性.1.1 理化方法 包括电穿孔,颗粒轰击技术,DNA直接注射,脂质载体法,磷酸钙沉淀法,DETE-葡聚糖法等。磷酸钙沉淀法尚能形成稳定的整合和长期高效表达,在一定条件下仍不失为一个较为简便和有效的方法.脂质载体法是在体内外均可使用的方法.往黑色素瘤瘤体内直接注射脂质体包裹的IL-2基因10天
In 1989 Tepper and other interleukin 4 (IL-4) gene into breast cancer cells found to be able to induce body-specific anti-tumor effect, which opened the prelude to the study of gene-based vaccines. Interleukin 2 (IL-2) Is the cytokine secreted by antigen-activated TH cells, plays an important role in the regulation of immune function in rabbits, especially in the regulation of anti-tumor immunity.In recent years, scholars at home and abroad have made a large amount of research on IL- In this paper, we review the progress.1 Gene transfer technology to transfect IL-2 gene into tumor cells and obtain high level of expression is the key technology of transgenic tumor vaccine.It is an ideal technology to make the target gene efficiently Introduction of human tumor cells, and easy to operate, with safety.1.1 Physical and chemical methods include electroporation, particle bombardment technology, DNA direct injection, lipid carrier method, calcium phosphate precipitation method, DETE-dextran method. Calcium phosphate precipitation method is still able to form a stable integration and long-term high expression, under certain conditions is still a relatively simple and effective method. Lipid vector method is available in vivo and in vitro methods. Into the melanoma tumor direct injection Liposome-encapsulated IL-2 gene for 10 days