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目的:研究Gαq/11在急性肺损伤(acute lung injury,ALI)大鼠脾脏的动态表达及意义。方法:40只Wister大鼠随机分为对照组(C组)和油酸组(OA组),后者用尾静脉注射油酸法复制ALI模型,并按不同时点分为30、60、90、120 min 4个亚组,免疫印迹法检测脾脏Gαq/11含量;检测血浆和脾脏组织的超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量。结果:与C组相比,OA组除OA 30 min外,Gαq/11含量随时间延长升高(P<0.05或0.01),血浆和脾脏的SOD活性降低(P<0.01),MDA含量增高(P<0.05或0.01);OA组PaO2、MAP逐渐降低(P<0.05或0.01)。结论:Gαq/11上调可能在ALI时脾脏受损中发挥一定作用,且其介导的信号转导系统的改变可能参与了ALI的发生发展。
Objective: To study the dynamic expression of Gαq / 11 in the spleen of rats with acute lung injury (ALI) and its significance. Methods: Forty Wister rats were randomly divided into control group (C group) and oleic acid group (OA group). The latter group were given ALO model by tail vein injection of oleic acid and divided into 30,60,90 , 120 subgroups for 4 min. The contents of Gαq / 11 in the spleen were detected by Western blotting. The activity of SOD and the content of malondialdehyde (MDA) in the plasma and spleen of the rats were detected. Results: Compared with group C, the content of Gαq / 11 in OA group increased with time (P <0.05 or 0.01), SOD activity in plasma and spleen decreased (P <0.01) and MDA content increased P <0.05 or 0.01). PaO2 and MAP in OA group decreased gradually (P <0.05 or 0.01). CONCLUSION: The up-regulation of Gαq / 11 may play a role in the pathogenesis of splenic injury during ALI. The changes of signal transduction system may be involved in the development of ALI.