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目的分析唐山地区女性人乳头瘤病毒(HPV)感染阳性率、基因分型及与宫颈病变的相关性。方法采用PCR以及线性杂交法(LA)对2 557例女性宫颈脱落细胞标本进行HPV检测和基因分型。结果 2 557例患者中HPV阳性675人,阳性率26.4%;20岁以下阳性率(66.7%)显著高于其余各组(P<0.05),20岁以上各组阳性率在20.9%~27.4%之间,组间差异无统计学意义(P>0.05);高危型以HPV16、58、52基因型为主,低危型以HPVCP8304、6、11基因型为主;HPV16在宫颈癌、宫颈上皮内瘤变(CIN)和慢性宫颈炎的阳性率分别为51.8%、67.6%和32.6%。结论唐山地区HPV感染的主要基因型与国内其他地区报道基本一致,HPV基因分型对该地区女性HPV感染前的免疫接种具有较好的指导意义。
Objective To analyze the positive rate, genotyping and cervical lesions of female human papillomavirus (HPV) in Tangshan area. Methods PCR and linear hybridization (LA) were used to detect HPV genotypes in 2 557 female cervical exfoliated cells. Results The positive rate of HPV was 675 in 2 557 patients, the positive rate was 26.4%. The positive rate was 66.7% in 20 years old (P <0.05), and the positive rate in 20 years old and above was 20.9% -27.4% (P> 0.05). High-risk genotypes were mainly HPV16, 58 and 52 genotypes, and low-risk genotypes were HPVCP8304,6 and 11 genotypes. HPV16 was mainly expressed in cervical cancer and cervical epithelium The positive rates of CIN and chronic cervicitis were 51.8%, 67.6% and 32.6% respectively. Conclusion The main genotypes of HPV infection in Tangshan area are basically the same as those reported in other areas in China. HPV genotyping has good guiding significance for pre-HPV vaccination in the area.