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为评价Na~+—H~+交换阻滞剂对缺血再灌注损伤的保护作用,在改良的Langendoff装置上采取主动脉逆灌方法,用成年Wistar大鼠的离体鼠心完全停灌60min,然后100％再灌注30min造成再灌注损伤模型。阿米洛利(0.2、20、100~μmol/L)和速尿(10~μmol/L)为药物保护组及不加药物的对照组。结果显示;阿米洛利和速尿对再灌注时心肌收缩力、冠脉流量的恢复及抑制再灌注心律失常的发生有明显的作用,阿米洛利抗心律失常的效果呈明显的剂量依赖性。因此提示具Na—H交换阻断作用的阿米洛利和速尿有明显的抗再灌注损伤的作用。
In order to evaluate the protective effect of Na ~ + -H ~ + exchange blocker on ischemia-reperfusion injury, aortic instillation was performed on a modified Langendoff apparatus. The isolated rat heart of adult Wistar rats was stopped for 60min , Followed by 100% reperfusion for 30 minutes to induce a reperfusion injury model. Amiloride (0.2,20,100 ~ μmol / L) and furosemide (10 ~ μmol / L) were drug-protective group and non-drug control group. The results showed that amiloride and furosemide had significant effects on myocardial contractility, coronary flow recovery and inhibition of reperfusion arrhythmia during reperfusion. The anti-arrhythmic effect of amiloride was significantly dose-dependent . Therefore, it is suggested that amiloride and furosemide with Na-H exchange blockade have significant anti-reperfusion injury effects.