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目的 探讨移植施万细胞 (SCs)和应用一氧化氮合酶抑制剂L 硝基精氨酸 (L NNA)能否促进脑干红核受损伤的神经元存活。 方法 2 0只成年SD雌性大鼠分为 :对照组、SCs组、L NNA组和SCs +L NNA组。在胸11脊髓段半横断后立即在损伤处植入SCs,术后腹腔注射L NNA。 结果 脊髓半横断后 30d ,对照组和L NNA组大鼠受损伤侧红核的神经元密度明显降低 ,但两组间无明显差异 ;两组受损伤侧红核神经元的NOS表达阴性。SCs组和SCs +L NNA组大鼠受损伤侧红核的神经元密度明显增加 ,两组间无显著差异 ;两组受损伤侧红核神经元NOS表达也呈阴性。 结论 移植的SCs能够促进受损伤的红核神经元存活。NOS可能不参与影响受损伤红核神经元的存活过程 ,因而L NNA对受损伤红核神经元的存活没有作用。
Objective To investigate whether transplanted Schwann cells (SCs) and nitric oxide synthase inhibitor L-nitro-arginine (L-NNA) can promote the survival of neurons injured in the nucleus accumbens of the brain. Methods Twenty adult SD female rats were divided into control group, SCs group, LNA group and SCs + LNA group. SCs were implanted in the lesion immediately after the transection of the thoracic 11 spinal cord and LNA was injected intraperitoneally after the operation. Results At 30 days after spinal cord transection, the density of neurons in injured red nucleus was significantly decreased in both control and LNA groups, but there was no significant difference between the two groups. NOS expression in injured lesioned nucleus neurons was negative in both groups. The density of neurons in injured SCs was significantly increased in SCs and SCs + L NNA groups, but there was no significant difference between the two groups. NOS expression was also negative in injured red nucleus. Conclusion Transplanted SCs can promote the survival of injured red nucleus neurons. NOS may not be involved in the process of affecting the survival of injured red nucleus neurons, and therefore L NNA has no effect on the survival of injured red nucleus neurons.