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目的:建立同时测定利咽气雾剂中脂蟾毒配基和华蟾酥毒基含量的方法。方法:采用RP-HPLC法,ZORBAX-C18柱(4.6mm×250mm,5μm),流动相为:乙腈-0.5%磷酸二氢钾溶液(55∶45)(磷酸调pH3.2),流速为0.8mL·min-1,柱温:30℃,检测波长为296nm。结果:脂蟾毒配基和华蟾酥毒基分别在5.15～103.00μg.mL-1(r=0.9994)和2.70～54.00μg.mL-1(r=0.9998)范围内均有良好的线性关系,平均加样回收率分别为98.72%,98.99%;RSD均小于2.0%。结论:本方法简便,快速,准确,重复性好,可用于该制剂的质量控制。
OBJECTIVE: To establish a method for the simultaneous determination of latoxifloxacin and toadine in Liyan’s aerosol. Methods: The mobile phase was acetonitrile-0.5% potassium dihydrogen phosphate solution (55:45) (adjusted to pH3.2 with phosphoric acid) by RP-HPLC on a ZORBAX-C18 column (4.6 mm × 250 mm, mL · min-1, column temperature: 30 ℃, detection wavelength of 296nm. Results: The results showed that there was a good linear relationship between ADP and CSD in the range of 5.15-103.00μg.mL-1 (r = 0.9994) and 2.70-54.00μg.mL-1 (r = 0.9998) The average recoveries were 98.72% and 98.99%, respectively; RSDs were less than 2.0%. Conclusion: The method is simple, rapid, accurate, reproducible and can be used for the quality control of the preparation.