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[目的]探讨细胞色素P450(A)(CYP1A1)的基因多态性、血清锌水平单独作用以及联合作用与非小细胞肺癌发生危险的关系。[方法]采用病例对照研究方法,病例78例,对照78例。用限制性片断长度多态性PCR技术(PCR-RFLP)检测CYP1A1的MspI多态性,用电感耦合等离定子体发射光谱法(ICP-AES)测血清锌水平。[结果]CYP1A1的MspI多态性单独作用时与肺癌危险性关系差异无统计学意义(P﹥0.05);病例组血清锌水平显著低于对照组(P﹤0.01);以血清锌≥117.0mg/dL且CYP1A1野生型基因携带者为参照组(OR=1.00),则血清锌﹤117.0mg/dL且携带CYP1A1突变型或杂合型或野生型基因者的OR分别为5.50(P﹤0.05)、10.63(P﹤0.01)、10.08(P﹤0.01)。[结论]CYP1A1基因多态性单独作用时NSCLC发生无显著相关;血清锌水平与NSCLC发生呈负相关;CYP1A1基因多态性与血清锌联合作用时明显提高NSCLC发生的危险性,在NSCLC发生中存在协同作用。
[Objective] To explore the relationship between genetic polymorphism of cytochrome P450 (A) (CYP1A1), serum zinc level alone and combined action and the risk of non-small cell lung cancer. [Method] The case-control study method, 78 cases, control 78 cases. The MspI polymorphism of CYP1A1 was detected by restriction fragment length polymorphism PCR (PCR-RFLP) and the level of serum zinc was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). [Results] There was no significant difference between the MspI polymorphism of CYP1A1 and the risk of lung cancer (P> 0.05). The level of serum zinc in the case group was significantly lower than that of the control group (P <0.01) / dL and CYP1A1 wild-type gene carriers as a reference group (OR = 1.00), OR of serum zinc <117.0 mg / dL and those carrying CYP1A1 mutant or heterozygous or wild-type genes were 5.50 (P <0.05) , 10.63 (P <0.01), 10.08 (P <0.01). [Conclusion] There is no significant correlation between CYP1A1 gene polymorphism and NSCLC. Serum zinc level is negatively correlated with NSCLC. CYP1A1 gene polymorphism significantly increases the risk of NSCLC when combined with serum zinc. In the occurrence of NSCLC There is synergy.