对于酚酸化合物的分离,过去多用正相薄层色谱法,但效果不甚好。近来国外学者开始采用反相薄层色谱及反相液相色谱分离,效果大大提高。本文使用两种方法,在反相高效色谱柱上完成了对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸,包括对香豆酸和阿魏酸异构体的分离,并研究了上述化合物的保留值与流动相中有机相比例的关系。 For the separation of phenolic compounds, the past use of normal phase thin layer chromatography, but the effect is not very good. Recently, foreign scholars began to use reverse-phase thin-layer chromatography and reverse-phase liquid chromatography separation, the effect is greatly enhanced. In this paper, two methods were used to separate p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid and ferulic acid, including p-coumaric acid and ferulic acid isomers on reversed- The relationship between the retention of the above compounds and the organic phase in the mobile phase was also studied.