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目的:观察藤茶提取物(TF)抗慢性肝纤维化的作用及机制。方法:将Wistar大鼠随机分为TF高(200 mg.kg-1)、中(100 mg.kg-1)、低(50 mg.kg-1)剂量组,秋水仙碱(colchicine,Colc,0.1 mg.kg-1)组,模型组和正常组,ig给药。给药同时各组皮下注射(sc)四氯化碳(CCl4)造模,每周2次。于10周后,检测大鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(AKP),白蛋白(ALB),球蛋白(GLB),肝组织羟脯氨酸(Hyp),丙二醛(MDA),谷胱甘肽过氧化物酶(GSH-Px)及苏木精-伊红(HE)染色后的肝组织病理变化。结果:与模型组比较,TF高、中、低剂量组均能明显降低肝纤维化大鼠血清中ALT,AST,AKP,GLB的含量(P<0.01)及肝组织中MDA和Hyp的含量(P<0.05),明显提高血清ALB水平(P<0.01)及肝组织GSH-Px活性(P<0.01);肝脏组织学检查表明,TF可明显改善肝组织病理损伤程度,其作用呈剂量依赖性。结论:TF对实验性慢性肝损伤具有保护肝细胞,减少肝损伤,抗肝纤维化作用。
Objective: To observe the anti-chronic liver fibrosis effect and mechanism of rattan tea extract (TF). Methods: Wistar rats were randomly divided into two groups: the control group (200 mg · kg -1), the medium (100 mg · kg -1) and the low dose (50 mg · kg -1) 0.1 mg.kg-1) group, model group and normal group, ig administration. Each group was subcutaneously injected (sc) with carbon tetrachloride (CCl4) twice a week for administration. After 10 weeks, serum ALT, AST, AKP, ALB, GLB, Hyp, MDA, GSH-Px and hematoxylin-eosin (HE) staining. Results: Compared with the model group, the levels of ALT, AST, AKP and GLB in the serum of the liver fibrosis rats (P <0.01) and the content of MDA and Hyp in the liver tissue of the rats in high, P <0.05), significantly increased serum ALB level (P <0.01) and hepatic GSH-Px activity (P <0.01). Liver histological examination showed that TF could significantly improve the degree of hepatic pathological damage, and its effect was dose-dependent . Conclusion: TF can protect liver cells, reduce hepatic injury and prevent hepatic fibrosis in experimental chronic liver injury.