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肉桂酰辅酶A还原酶 (CCR)负责催化木质素单体生物合成中最重要的代谢反应 ,它将类苯丙酸类代谢物转移到木质素的合成途径中。为了更好地了解木质素在小麦生长发育中的作用 ,从小麦 (TriticumaestivumL .cv .H4 5 64)中克隆了两个肉桂酰辅酶A还原酶的cDNA。相似性和进化关系的分析表明这两个cDNA片段分别属于不同的肉桂酰辅酶A还原酶 ,这两个cDNA片段分别命名为W_cr6和W_cr19。RT_PCR和Northern杂交结果证明 ,W_cr6基因主要在小麦的茎和叶中表达 ,W_cr19基因主要在根和茎中表达。上述结果表明在小麦的基因组中至少存在两类肉桂酰辅酶A还原酶基因。
Cinnamyl Coenzyme A Reductase (CCR) is responsible for catalyzing the most important metabolic reactions in lignin monomer biosynthesis, which convert the phenylalanine metabolites to the lignin synthesis pathway. To better understand the role of lignin in wheat growth and development, cDNA of two cinnamoyl-CoA reductase genes was cloned from wheat (Triticum aestivum L. cv. H4 5 64). Analysis of the similarities and evolutionary relationships indicated that the two cDNA fragments belonged to different cinnamoyl-CoA reductases, respectively, and the two cDNA fragments were named W_cr6 and W_cr19, respectively. The results of RT-PCR and Northern blot showed that the Wcr6 gene was mainly expressed in the stem and leaf of wheat and the Wcr19 gene was mainly expressed in the root and stem. The above results indicate that at least two types of cinnamoyl-CoA reductase genes are present in the genome of wheat.