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为探讨乙型肝炎病毒 (HBV)重叠丙型肝炎病毒 (HCV)感染时 HCV对 HBV复制和基因变异的影响 ,采用套式聚合酶链反应 (PCR)与限制性片段长度多态性 (RFLP)相结合 ,对 1 9例 HBV感染重叠 HCV感染 (A组 )和 31例单独 HBV感染 (B组 )的慢性肝病患者分析前 C区密码 2 8终止变异(A83)和 C区密码 97异亮氨酸变为亮氨酸变异 (L97)。结果显示 A组第一次 PCR阳性率 (1 6% )明显低于 B组 (65% ) (P<0 .0 0 1 ) ,A组 L97和 A83检出率分别为 35%和 2 8% ,明显低于 B组的 57%和 68%(P<0 .0 0 1 )。结果提示 :慢性乙型肝炎重叠 HCV感染 ,HCV对 HBV复制有抑制作用 ,HBV发生前 C/C基因变异的频率低
To investigate the effect of HCV on HBV replication and genetic variation in hepatitis C virus (HBV) overlapping hepatitis C virus (HCV) infection, polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) (A83) and C-region code 97 iso-amylamine were analyzed in 19 patients with chronic liver disease with overlapping HCV infection (group A) and 31 individuals with HBV infection alone (group B) Acid changed to leucine variant (L97). The results showed that the positive rate of PCR in group A (16%) was significantly lower than that in group B (65%) (P <0.01). The detection rates of L97 and A83 in group A were 35% and 28% , Which was significantly lower than 57% and 68% in group B (P <0. 001). The results suggest that HCV infection has an inhibitory effect on HCV replication in patients with chronic hepatitis B. The frequency of C / C gene mutation before HBV infection is low