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目的 探讨转化生长因子 β(TGF- β)蛋白及 m RNA基因在系膜增生性肾小球肾炎中的表达及其在发病中的作用。方法 采用常规病理、免疫组织化学及原位杂交方法对人系膜增生性肾小球肾炎组织进行染色 ,并经医学图像分析系统进行分析。结果 在正常对照组 ,TGF- β蛋白及 TGF- β1m RNA于肾小管上皮细胞呈极微弱表达 ,肾小球内 TGF- β蛋白及m RNA未见阳性表达。在系膜增生性肾小球肾炎组织中 ,TGF- β蛋白在肾近曲小管上皮细胞胞浆内呈强阳性表达 ,肾小球球囊壁及系膜区呈阳性表达 ,肾小球与肾小管阳性表达与正常对照组相比均具有显著性差异 (P<0 .0 1)。TGF- β1m RNA阳性表达位于肾近曲小管上皮细胞胞浆内和肾小球系膜区 ,肾小球和肾小管上皮细胞 TGF- β1m RNA阳性表达与正常对照组相比差异也有显著性 (P<0 .0 1)。结论 系膜增生性肾小球肾炎时 TGF- β蛋白及 TGF- β1m RNA在肾小球与肾小管表达均显著增高 ,进一步显示 TGF- β在肾小球系膜细胞增生及肾小管间质纤维化中所起的重要作用。
Objective To investigate the expression of transforming growth factor β (TGF-β) and m RNA in mesangial proliferative glomerulonephritis and its role in the pathogenesis. Methods Human mesangial proliferative glomerulonephritis was stained by routine pathology, immunohistochemistry and in situ hybridization and analyzed by medical image analysis system. Results In the normal control group, TGF-β protein and TGF-β1m RNA were very weakly expressed in renal tubular epithelial cells, and no expression of TGF-β protein and m RNA in glomeruli was found. In mesangial proliferative glomerulonephritis tissues, TGF-βprotein was strongly positive in the cytoplasm of renal proximal tubule epithelial cells, with positive expression in glomerular balloon wall and mesangial area. Glomerular and renal Tubular expression was significantly different from the normal control group (P <0.01). The positive expression of TGF-β1mRNA in renal proximal tubule epithelial cells in the cytoplasm and mesangial area, glomerular and renal tubular epithelial cells TGF-β1mRNA positive expression compared with the normal control group, the difference was significant (P <0 .0 1). Conclusion The expression of TGF-β and TGF-β1mRNA in glomeruli and tubules in mesangial proliferative glomerulonephritis is significantly increased, which further shows that TGF-β is involved in mesangial cell proliferation and tubulointerstitial fibrosis The important role played by.