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采用免疫组织化学方法观察了重组人白细胞介素-1β、重组人白细胞介素-2和重组人白细胞介素-6对体外培养大鼠海马神经胶质细胞c-fos表达的影响。结果证明,培养12d的海马神经胶质细胞与重组人白细胞介素-1β(100U/ml)、重组人白细胞介素-2(200U/ml)和重组人白细胞介素-(200U/ml)一起孵育2h后出现FOS-免疫反应阳性的细胞核,随着所给剂量的逐渐加大,FOS反应阳性胞核数量也逐渐增多.图象分析的结果显示,FOS反应阳性胞核的平均光密度亦随剂量的逐渐加大而逐渐增加.本研究的结果提示:重组人白细胞介素-1β、-2及-6均能诱导体外培养大鼠海马神经胶质细胞c-fos的表达。说明三者对体外培养的海马神经胶质细胞具有生物学作用。推测c-fos表达可能是这些细胞因子发挥生物效应的重要中间途径。
The effect of recombinant human interleukin-1β, recombinant human interleukin-2 and recombinant human interleukin-6 on c-fos expression in cultured rat hippocampal neurons was observed by immunohistochemistry. The results showed that hippocampal neurons cultured with recombinant human interleukin-1β (100U / ml), recombinant human interleukin-2 (200U / ml) and recombinant human interleukin- (200U / ml) Fos-immunoreactive nuclei appeared after 2h incubation, and the number of FOS positive nuclei gradually increased with the dose increasing. The results of image analysis showed that the average optical density of FOS positive nuclei also gradually increased with the dose gradually increased. The results of this study suggest that recombinant human interleukin-1β, -2 and -6 can induce c-fos expression in cultured rat hippocampal neurons. All three showed biological effects on cultured hippocampal glial cells in vitro. It is speculated that c-fos expression may be an important intermediate pathway for these cytokines to exert biological effects.