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目的：研究胰腺癌中 Ｐ１６和 Ｐ５３基因产物的表达及其临床意义。方法：应用免疫组化 Ｌ Ｓ Ａ Ｂ法研究２８例胰腺癌组织中 Ｐ１６蛋白和 Ｐ５３蛋白的表达情况，并进行增殖细胞核抗原（ Ｐ Ｃ Ｎ Ａ）检测，计算细胞增殖指数（ Ｐｒｏｌｉｆｅｒａｔｉｏｎ ｉｎｄｅｘ， Ｐ Ｉ）。结果： Ｐ１６蛋白低表达和 Ｐ５３蛋白过表达均有促进胰腺癌细胞增殖的作用。 Ｐ１６蛋白表达与淋巴结转移无关； Ｐ５３蛋白高表达者具有较高的淋巴结转移率，预后较差。 Ｐ１６蛋白低表达与 Ｐ５３蛋白高表达共存者，其恶性度最高，预后最差。结论：联合检测 Ｐ１６和 Ｐ５３蛋白表达情况，更能充分反映胰腺癌的生物学行为，对胰腺癌患者预后判断及临床治疗有指导意义。
Objective: To study the expression and clinical significance of P16 and P53 gene products in pancreatic cancer. METHODS: The expression of P16 protein and P53 protein in 28 cases of pancreatic cancer was studied by immunohistochemical L S A B method. Proliferation cell nuclear antigen (PCNA) was detected and the cell proliferation index was calculated (Proliferation index, P I ). RESULTS: Low expression of P16 protein and overexpression of P53 protein all promoted the proliferation of pancreatic cancer cells. P16 protein expression was not associated with lymph node metastasis. High expression of P53 protein had higher lymph node metastasis rate and poorer prognosis. Co-existence of low expression of P16 protein and high expression of P53 protein has the highest malignancy and the worst prognosis. Conclusion: Combined detection of P16 and P53 protein expression can fully reflect the biological behavior of pancreatic cancer, and it has guiding significance for prognosis and clinical treatment of pancreatic cancer.