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目的:建立人肺癌小鼠高转移模型及高转移细胞系,同时观察相关生物学特性,为肺癌转移机制和防治等研究提供有用的实验工具。方法:切除首代小鼠移植瘤,以延长动物生存时间而获得转移灶,从第二代起采用肺转移灶→皮下移植→肺转移灶→皮下移植的体内循环筛选方法建立NOD/SCID小鼠人肺癌细胞SPC-A-1皮下移植瘤高转移模型,并进行肿瘤的生长和转移情况、组织病理学观察,同时建立相应的高转移细胞系,进行各种相关生物学特性观察。结果:第一代移植瘤切除后转移率达66.7%,通过4代体内反复筛选建立了100%肺转移NOD/SCID小鼠模型及相应高转移细胞系,细胞生长行为和染色体分析等生物学特性观察表明该细胞系保持了原有的人肺腺癌的生物学特性。结论:应用体内筛选的方法成功建立了人肺癌皮下移植瘤高转移模型及高转移细胞系,为肺癌防治研究及抗转移实验治疗提供了理想的动物模型。
OBJECTIVE: To establish a human lung cancer mouse model of high metastasis and high metastatic cell lines, and to observe the relevant biological characteristics, and to provide a useful experimental tool for lung cancer metastasis mechanism and prevention and treatment. Methods: The first generation of mouse xenografts was excised to prolong the survival time of the animals to obtain metastatic tumors. From the second generation, NOD / SCID mice were established by in vivo circulation screening of lung metastasis, subcutaneous transplantation, lung metastasis and subcutaneous transplantation The human lung cancer cell SPC-A-1 subcutaneously transplanted tumor high metastasis model, and tumor growth and metastasis, histopathological observation, and the establishment of the corresponding high-metastatic cell line, the observation of a variety of relevant biological characteristics. RESULTS: The first generation of xenotransplanted tumors achieved a metastasis rate of 66.7% after resection. The biological characteristics of 100% lung metastatic NOD / SCID mouse model and corresponding highly metastatic cell lines, cell growth behavior and chromosome analysis were established by repeated in vivo screening in the fourth generation Observations show that the cell line maintains the biological characteristics of the original human lung adenocarcinoma. Conclusion: The high metastasis model of human lung cancer subcutaneously transplanted tumor and its highly metastatic cell line were successfully established by in vivo screening method, which provided an ideal animal model for lung cancer prevention and treatment and anti-metastasis experimental treatment.