论文部分内容阅读
目的:探讨不同血流阻断方式对荷瘤小鼠肝细胞功能的影响。方法:选择昆明小鼠24只随机分为三组,正常对照组(Suspe-nded operation,SO)、肝门阻断组(Occlusion of the portal triad,OPT)、保留肝动脉持续阻断门静脉(Occlusion of portal vein,OPV)各8只。采用门静脉注射肿瘤的方法建立肝癌模型,建模后3天采用阻断范围为左外叶和中叶、阻断时间为60分钟的入肝血流阻断方式,复流后5天后,通过测量3组对肝脏的缺血再灌注损伤程度以及病理学变化来评价不同血流阻断方式对肝细胞功能影响的程度。结果:门静脉注射小鼠肝癌细胞8天后,对照组测量小鼠正常丙氨酸氨基转移酶(ALT)值为66.5±22.3 IU/L,OPT组值为276.3±80.5 IU/L,OPV组值为89.6±28.4 IU/L,两组比较有统计学差异(P<0.01);对照组测量小鼠正常天冬氨酸氨基转移酶(AST)值为301.3±126.7 IU/L,OPT组值为1126.4±285.5 IU/L,OPV组值为438.6±150.7 IU/L,两组比较有统计学差异(P<0.01),病理组织学OPV组肝细胞损伤程度明显较OPT组轻。结论:保留肝动脉持续阻断门静脉可以减轻荷瘤小鼠肝脏的缺血再灌注损伤。
Objective: To investigate the effect of different blocking methods on hepatocyte function in tumor-bearing mice. Methods: Twenty-four Kunming mice were randomly divided into three groups: the control group (SO), the occlusion of the portal triad (OPT), the occlusion of the hepatic artery (Occlusion of portal vein, OPV). The model of hepatocellular carcinoma was established by portal vein injection of tumor. After 3 days of modeling, the occlusion of left hepatic lobe and middle lobe was performed. The occlusion time was 60 minutes. After 5 days of recuperation, Group of liver ischemia-reperfusion injury and pathological changes to evaluate the different ways of blocking blood flow on the degree of hepatocyte function. Results: After 8 days of portal vein injection of mouse hepatoma cells, normal ALT was 66.5 ± 22.3 IU / L in the control group, 276.3 ± 80.5 IU / L in the OPT group, and OPV group was 89.6 ± 28.4 IU / L, the difference between the two groups was statistically significant (P <0.01); the control group measured normal aspartate aminotransferase (AST) values of 301.3 ± 126.7 IU / L, OPT group was 1126.4 ± 285.5 IU / L, the OPV group was 438.6 ± 150.7 IU / L, the difference between the two groups was statistically significant (P <0.01). The degree of hepatocellular injury in OPV group was significantly lower than that in OPT group. Conclusion: Keeping the hepatic artery and blocking the portal vein can reduce the ischemia-reperfusion injury in the liver of tumor-bearing mice.