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目的：进一步探索重组基因工程抗体在原核系统中有效的活性表达途径。方法：在已构建的抗ＨＢｘ单链抗体（ＳｃＦｖ）的基础上，利用粘粒载体构建了抗ＨＢｘ单链噬菌体抗体（ｐｈａｇｅａｎｔｉｂｏｄｙ），并转化大肠杆菌。结果：该噬菌体抗体经辅助噬菌体Ｍ１３ＫＯ７诱导和噬菌体次要衣壳蛋白（ＰⅢ）融合表达，并组装成噬菌体衣壳蛋白可溶性表达于培养液上清，经ＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ的结果证实可溶性表达产物具有ＨＢｘ抗原的特异性亲和力。结论：单链噬菌体抗体的构建表达成功，可望成为原核系统中表达筛选特异性抗体可变区及有效制备活性基因工程抗体的简捷途径
OBJECTIVE: To further explore the effective active expression of recombinant genetic engineering antibodies in prokaryotic system. Methods: Based on the constructed anti-HBx single-chain antibody (ScFv), an anti-HBx single-phage phage antibody was constructed with cosmid vector and transformed into Escherichia coli. Results: The recombinant phage antibody was induced by phage display M13KO7 fusion with the phage minor capsid protein (P Ⅲ), and then was assembled into phage-type capsid protein which was expressed in the supernatant of the culture medium. The results of ELISA and Western blot confirmed that the soluble expression product Has the specific affinity of HBx antigen. Conclusion: The construction and expression of single-chain phage antibody is successful, which is expected to be a convenient way to express the variable region of specific antibody and prokaryotic antibody in prokaryotic system