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研究蛋白酶酶促水解制备生物活性肽的反应机理与动力学行为,并在假设胰蛋白酶恒温控制水解动力学遵循内切酶限制水解动力学历程的前提下,通过实验方法求出了胰蛋白酶恒温控制水解动力学模型。结果表明,胰蛋白酶对鳕鱼皮胶原蛋白进行控制水解的动力学模型为:反应速率(R)=(14.231E0-1.745S0)exp[-0.514(DH)],水解度(DH)=1.946ln[1+(7.315E0/S0-0.897)t],并求得该体系反应速率常数K2=14.231 min-1,酶失活常数Kd=18.809 min-1。验证实验证明,根据胰蛋白酶恒温控制水解动力学模型得到的理论水解度与实际水解度基本吻合,所建模型可用于胶原蛋白酶解反应过程的模拟和酶解反应条件的优化设计。
The reaction mechanism and kinetic behavior of enzymatic hydrolysis of protease to prepare bioactive peptides were studied. Under the premise that trypsin thermostatically controlled hydrolysis kinetics followed the restriction endonuclease hydrolysis kinetics, trypsin thermostat control Hydrolytic kinetic model. The results showed that the kinetic model of controlled hydrolysis of cod skin collagen by trypsin was as follows: reaction rate (R) = (14.231E0-1.745S0) exp [-0.514 (DH)], degree of hydrolysis (DH) = 1.946ln [ 1 + (7.315E0 / S0-0.897) t]. The reaction rate constant K2 = 14.231 min-1 and the enzyme inactivation constant Kd = 18.809 min-1 were obtained. Validation experiments show that the theoretical hydrolysis degree obtained from the kinetic model of trypsin thermostatic control is in good agreement with the actual degree of hydrolysis, and the model can be used to simulate the collagenase reaction process and optimize the enzymatic hydrolysis conditions.