论文部分内容阅读
目的:探讨高糖对H9c2细胞凋亡及表达miR-34a的影响。方法:采用高糖(25mmol/L)刺激H9c2细胞(实验组),并设空白对照(对照组)。采用Tunel法和流式细胞仪检测细胞凋亡,采用定量PCR检测miR-34a、Bcl-2和Caspase-3基因表达的变化,Western blotting检测凋亡相关蛋白Bcl-2和Caspase-3的蛋白表达。结果:实验组H9c2细胞凋亡率较对照组明显增加[(10.47±0.21)%∶(6.21±0.02)%,P<0.05];定量PCR结果显示,实验组miR-34a表达显著上调[(0.98±0.09)∶(2.54±0.42),P<0.05];定量PCR和Western blotting结果显示,与对照组比较,实验组Bcl-2基因及蛋白表达明显降低,Caspase-3基因及蛋白表达显著增加(均P<0.05)。结论:高糖刺激可诱导心肌细胞凋亡,同时可调节与凋亡密切相关的miR34-a的表达。
Objective: To investigate the effect of high glucose on apoptosis and expression of miR-34a in H9c2 cells. Methods: H9c2 cells were stimulated with high glucose (25 mmol / L) (experimental group) and blank control group (control group). The apoptosis was detected by Tunel method and flow cytometry. The expression of miR-34a, Bcl-2 and Caspase-3 was detected by quantitative PCR. The protein expressions of Bcl-2 and Caspase-3 were detected by Western blotting . Results: The apoptosis rate of H9c2 cells in the experimental group was significantly higher than that in the control group [(10.47 ± 0.21)% vs (6.21 ± 0.02)%, P <0.05]. The quantitative PCR results showed that the expression of miR-34a in the experimental group was significantly increased ± 0.09): (2.54 ± 0.42), P <0.05]. The results of quantitative PCR and Western blotting showed that compared with the control group, the expression of Bcl-2 gene and protein was significantly decreased and the expression of Caspase-3 gene and protein were significantly increased All P <0.05). Conclusion: High glucose stimulation can induce cardiomyocyte apoptosis and regulate the expression of miR34-a, which is closely related to apoptosis.