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介绍了高效液相色谱在双酶协同作用酶解制取麦芽低聚糖工艺研究中的应用。以C18柱为分离柱，水作流动相，利用折光检测器来检测麦芽低聚糖产品中的7种糖，同时评估了补加酶量与麦芽低聚糖中麦芽三糖至六糖含量的关系，并测定了二次确认实验中麦芽低聚糖产品中各糖的含量。
The application of high performance liquid chromatography (HPLC) in the process of enzymatic hydrolysis of maltooligosaccharides by a double enzyme was introduced. Using C18 column as the separation column and water as the mobile phase, the refractive index detector was used to detect the seven kinds of sugars in the malt oligosaccharide products. The content of maltotriose to hexose in malt oligosaccharides Relationship and determined the content of each sugar in the malt oligosaccharide product in the secondary confirmation experiments.