人体肌活检培养法:肌肉活检:普鲁卡因局麻下取材,注意不要将麻药注入肌肉内。取小指头大小肌肉片,放入装有培养液的试管中。培养前放室温或4℃冰箱保存。培养方法:在无菌室内无菌操作。先将肌组织移至玻璃皿,在实体显微镜下尽可能地除去结缔组织及血管等。然后用刮脸刀片将肌组织切成约1mm~3的肌肉细片,将4～5个细片放在涂布了大白鼠尾部胶原的盖玻璃上,放在塑 Human muscle biopsy culture: muscle biopsy: procaine drawn under local anesthesia, be careful not to inject anesthetic into the muscle. Take the small finger size muscle piece, into the test tube with culture medium. Pre-incubation room temperature or 4 ℃ refrigerator preservation. Training methods: aseptic room sterile operation. Muscle tissue first move to the glass dish, as much as possible under the physical microscope to remove connective tissue and blood vessels. Then use the shaving blade muscle tissue cut into about 1mm ~ 3 muscle fragments, the 4 to 5 tablets coated on the tail of the mouse coated collagen cover glass, plastic