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了解中国福建省龙岩市2011~2014年病毒性脑炎散发病例中Echo 30分子流行病学特征。收集病毒性脑炎或中枢神经系统感染脑脊液标本,细胞培养分离病毒,RT-PCR扩增VP1基因序列全长以鉴定Echo 30血清型及遗传特征分析。2011~2014年共从608例监测病例中分离到168株肠道病毒,其中60株为Echo 30;60例Echo 30相关病例分析显示,Echo 30流行高峰为6~8月;波及年龄范围广,以小于10岁高发(65%);临床症状以发热、头痛和呕吐为主,脑脊液清,细胞和蛋白含量检测多增加。VP1区分析显示,2011~2014年龙岩流行株均属于h基因型,但有两个传播链共同循环;与2011年导致福建省病毒性脑炎暴发毒株高度同源,但2014年龙岩株均在VP1蛋白I 120V出现新氨基酸变异。福建省2011年暴发流行株仍流行于龙岩市,且两个传播链病毒仍共同循环,但2014年病毒株出现了新的变异,持续监测将有利于早期发现病毒变异积累和评估疾病流行的风险。
To understand the molecular epidemiology of Echo 30 in cases of encephalitis eradication in Longyan, Fujian Province, China. Cerebrospinal fluid samples were collected from patients with viral encephalitis or CNS infection. Virus was isolated from the cells and the full-length VP1 gene was amplified by RT-PCR to identify the Echo 30 serotypes and genetic characteristics. From 2011 to 2014, a total of 168 enteroviruses were isolated from 608 surveillance cases, of which 60 were Echo 30. The analysis of 60 cases of Echo 30 showed that the peak of Echo 30 was from June to August and the range of age, High incidence of less than 10 years old (65%); clinical symptoms of fever, headache and vomiting, cerebrospinal fluid clear, more detection of cellular and protein levels. The analysis of VP1 showed that the epidemic strains of Longyan from 2011 to 2014 belonged to genotype h but had two circulating chains that circulated together. In 2011, the genotypes of Longyan strains were highly homologous to the strains of virulent encephalitis in Fujian Province. However, A new amino acid variation occurs at VP1 protein I 120V. The outbreak strains of Fujian Province still prevailed in Longyan City in 2011, and the two transmitted chain viruses still co-circulated. However, new mutations appeared in the strain in 2014 and continuous monitoring would be conducive to detecting the early accumulation of virus variation and assessing the risk of the epidemic .