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作者将成骨细胞系MC3T3-E_1接种到各种材料上,并加入含10％FCS的DMEM,在37℃,5％CO_2条件下培养,从培养1 h至10 d分批制备标本,进行扫描
The osteoblast cell line MC3T3-E_1 was inoculated on various materials and cultured in DMEM containing 10% FCS at 37 ℃ under 5% CO 2. The samples were prepared in batches from 1 h to 10 d after culture.