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目的观察不同剂量氟哌啶醇对新西兰兔心电图QT间期的影响及其对L型钙通道mRNA表达水平的影响。方法采用体表心电图技术,通过BL-410生物机能实验系统动态记录Ⅱ导联心电图的变化,观察不同剂量氟哌啶醇对新西兰兔心电图的影响。提取心肌组织总RNA,采用RT-PCR方法,观察氟哌啶醇对L型钙通道mRNA表达水平的影响。结果氟哌啶醇能引起的QT间期延长,剂量越大延长越明显。氟哌啶醇对QT间期的影响主要发生在给药后0～120min,360min后基本恢复正常。RT-PCR结果显示氟哌啶醇使L型钙通道mRNA表达水平明显增高。结论氟哌啶醇可使QT间期延长,其机制可能与增强L型钙通道mRNA表达有关,从而增加发生室性心律失常的潜在危险性。
Objective To observe the effects of different doses of haloperidol on the QT interval of ECG in New Zealand rabbits and its effect on the expression of L-type calcium channel mRNA. Methods The changes of electrocardiogram of Ⅱ lead were recorded dynamically by BL-410 bio-functional experimental system by surface electrocardiogram. The effects of different doses of haloperidol on ECG of New Zealand rabbits were observed. Total RNA was extracted from myocardium. The effects of haloperidol on the mRNA expression of L-type calcium channels were observed by RT-PCR. Results haloperidol can cause prolonged QT interval, the greater the extension of the more obvious dose. The effect of haloperidol on QT interval occurred mainly from 0 to 120 min after administration and returned to normal after 360 min. RT-PCR results showed that haloperidol significantly increased the expression of L-type calcium channel mRNA. Conclusions Haloperidol can prolong the QT interval, and its mechanism may be related to the enhancement of L-type calcium channel mRNA expression, thereby increasing the potential risk of developing ventricular arrhythmia.