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目的 通过比较正常孕妇与妊娠高血压综合征(妊高征)患者胎盘分泌的血管生长因子,包括血管内皮生长因子、碱性成纤维细胞生长因子及血小板来源生长因子的表达变化,探讨血管生长因子在妊高征的表达及意义。 方法 采集正常孕妇(15例)及妊高征患者(19例)的胎盘组织,采用免疫组织化学PAP法结合计算机显微图像分析软件,检测胎盘血管生长因子的表达。 结果 妊高征组中,中、重度妊高征患者胎盘的血管内皮生长因子阳性反应平均灰度值为 61.6±3.7,明显低于正常妊娠组的71.1±5.8和轻度妊高征的70.9±8.9,差异有统计学意义(P<0.01);碱性成纤维细胞生长因子阳性反应平均灰度值为 60.9±8.8,较正常妊娠组的 79.7±7.6 和轻度妊高征的78.4±10.4明显下降,差异有统计学意义(P<0.01);血小板来源生长因子阳性反应平均灰度值为37.5±5.6,较正常妊娠组的22.1±3.1和轻度妊高征的 21.8±4.3 明显增加,差异亦有统计学意义(P<0.01)。 结论 胎盘血管生长因子表达异常,可能导致胎盘血管面积下降,胎盘血管壁张力增高、痉挛,从而影响胎盘的血液供应,导致妊高征的一系列病理生理改变;表达异常的程度与妊高征病情轻重有关。
Objective To compare the changes of placental secretion of vascular growth factors, including vascular endothelial growth factor, basic fibroblast growth factor and platelet-derived growth factor in normal pregnant women and pregnancy-induced hypertension syndrome (PIH) The expression and significance of PIH. Methods The placenta tissues of normal pregnant women (15 cases) and pregnancy induced hypertension patients (19 cases) were collected. The expression of placental vascular growth factor was detected by immunohistochemical PAP combined with computer image analysis software. Results The mean gray value of placental vascular endothelial growth factor positive rate in PIH group was 61.6 ± 3.7, significantly lower than that in normal pregnancy group (71.1 ± 5.8) and mild PIH (70.9 ± 8.9, the difference was statistically significant (P <0.01). The average gray value of positive rate of basic fibroblast growth factor was 60.9 ± 8.8, which was significantly higher than that of normal pregnancy group (79.7 ± 7.6) and mild pregnancy-induced hypertension syndrome (78.4 ± 10.4) (P <0.01). The average gray value of platelet-derived growth factor positive reaction was 37.5 ± 5.6, which was significantly higher than that of normal pregnancy group (22.1 ± 3.1) and mild pregnancy-induced hypertension syndrome (21.8 ± 4.3), the difference was statistically significant Also statistically significant (P <0.01). Conclusion Abnormal expression of placental vascular growth factor may lead to decreased placental vascular area, increased placental vascular wall tension, spasm, and thus affect the placental blood supply, leading to a series of pathophysiological changes in PIH; the degree of abnormal expression and pregnancy-induced hypertension Weight related.