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对采自额尔齐斯河的银鲫(Carassius auratus gibelio Bloch)、东方欧鳊(Abramis brama orientalis Berg)、黏鲈(Gymnocephalus cernua Linnaeus)、金鳟(Oncorhynchus mykiss Walbaum)感染的三代虫(Gyrodactylus sp.)进行了研究,通过几丁质结构的形态测量与比较,初步鉴定寄生于银鲫的为细锚三代(G.sprostonae),寄生东方欧鳊的为秀丽三代虫(G.elegans),寄生黏鲈的为普氏三代虫(G.prostate),寄生金鳟的为细鳞鲑三代虫(G.brachymystacis)。同时将测定的三代虫的ITS序列,与Gen Bank上三代虫序列进行比对分析,发现上述4种三代虫分别与细锚三代虫、秀丽三代虫、细鳞鲑三代虫、普氏三代虫的ITS同源性都在99.3%以上,进一步验证了形态学的鉴定结果。系统发育分析显示细鳞鲑三代虫和细锚三代虫位于G.(Limnonephrotus)亚属的一枝,秀丽三代虫和普氏三代虫位于G.(Gyrodactylus)亚属的一枝,而此两亚属亲缘关系较远。
The results showed that Gyrodactylus sp. Infected by Carassius auratus gibelio Bloch, Abramis brama orientalis Berg, Gymnocephalus cernua Linnaeus, Oncorhynchus mykiss Walbaum collected from the Irtysh River, .) Were studied. Through the morphological measurement and comparison of the chitin structure, we initially identified G.sprostonae, the third generation of G. gypsophila parasitic on silver crucian carp, G.elegans, The G. priestate for the sea bass and the G. brachymystacis for the parasitic trout. At the same time, the ITS sequences of the three generations of pests were compared with those of the third generation in Gen Bank. It was found that the above three kinds of three generations of pests were respectively associated with three generations of fine anchors, three generations of beauty, ITS homology was above 99.3%, further verifying the morphological identification results. Phylogenetic analysis showed that the three generations of Brassica oysters and the three generations of fine anchors were located in one branch of the genus G. (Limnonephrotus), while the three generations of the three generations of Beauty and Third Placenta belonged to a subgenus of G. (Gyrodactylus) The relationship is farther.