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[目的]探讨甜荞、苦荞和大野荞的高分子量种子蛋白亚基。[方法]利用SDS-PAGE电泳法对18个不同地方居群的大野荞、3个甜荞和3个苦荞收集系的高分子量种子蛋白亚基进行研究。[结果]从这3个物种的24个收集系中,在高分子量区域(20~70 kDa)共发现12个不同的种子蛋白亚基谱带,种间差异大,种内差异小。3个物种在种子蛋白亚基分布上存在一定的相似性,约30%的谱带相同。其中,甜荞发现有9个谱带,含1个独特谱带(SSP33.71);苦荞有8个谱带,含不同于甜荞的2个独特谱带(SSP39.15和SSP29.35);大野荞有7个谱带,不仅含有苦荞的独特谱带,而且其谱带分布与苦荞极为相似。[结论]该研究为荞麦品质遗传育种、种间系统关系、高品质种子蛋白亚基基因工程等研究提供指导。
[Objective] The aim was to discuss the high molecular weight seed protein subunits of buckwheat, tartary buckwheat and wild buckwheat. [Method] SDS-PAGE electrophoresis method was used to study the molecular weight subunits of high molecular weight seeds of 18 wild buckwheat, 3 sweet buckwheat and 3 tartary buckwheat collected from 18 different places. [Result] Twelve different seed protein subunit bands were found in high molecular weight region (20 ~ 70 kDa) from 24 collection lines of these 3 species, with large differences among species and small intraspecific difference. There were some similarities in the distribution of seed protein subunits among the three species, with about 30% of the same bands. Among them, nine bands were found in sweet buckwheat, including one unique band (SSP33.71); there were eight bands in buckwheat with two distinct bands (SSP39.15 and SSP29.35 ); There are seven bands of wild buckwheat, not only contains a unique band of buckwheat, but its distribution is very similar with the buckwheat. [Conclusion] The research provided guidance for the research on buckwheat quality genetic breeding, interspecific relationship and high quality seed protein subunit gene engineering.