论文部分内容阅读
①目的 探讨扇贝多肽 (PCF)对紫外线损伤Hela细胞的影响。②方法 培养 2 4h的Hela细胞 ,制备细胞悬液 (5× 1 0 8/L) ,分别置 2 4孔板 ,每孔 1mL。随机分为对照组 ,紫外线损伤模型组 ,5 g/LPCF组 ,1 0 g/LPCF组 ,2 0g/LPCF组和 1 0 g/L维生素C组。每组 3个复孔 ,分别在RPMI 1 6 4 0、PCF、维生素C孵育 1 0min后 ,分别置紫外线下照射 ,照射强度为UVA 36 5 0 μJ/cm2 、UVB 7.1 5× 1 0 -5J/cm2 。用流式细胞仪测定各组细胞的死亡率、凋亡率及细胞内Ca2 + 含量。用生物化学方法测定上清液中MDA含量和抗氧化酶的活性。③结果PCF可以降低紫外线损伤Hela细胞的死亡率和凋亡率 ,并且提高细胞内Ca2 + 含量和琥珀酸脱氧酶的活性。PCF还可降低MDA的含量 ,提高抗氧化酶GSH px、SOD和CAT的活性。 ④结论 PCF可能通过提高抗氧化酶的活性而发挥对紫外线损伤Hela细胞的保护作用
1 Objective To investigate the effect of scallop peptide (PCF) on ultraviolet damage Hela cells. 2 Methods Hela cells were cultured for 24 hours to prepare cell suspensions (5×108/L), which were placed in 24 well plates with 1 mL per well. They were randomly divided into control group, UV injury model group, 5 g/LPCF group, 10 g/LPCF group, 20 g/LPCF group and 10 g/L vitamin C group. Each group of three duplicate wells, respectively, in RPMI 1640, PCF, vitamin C incubated for 10 min, respectively, under ultraviolet irradiation, irradiation intensity of UVA 36 50 μJ/cm2, UVB 7.1 5× 1 0 -5J/ Cm2. Flow cytometry was used to determine the mortality, apoptotic rate and intracellular Ca2+ content of each group of cells. Biochemical methods were used to determine the supernatant MDA content and antioxidant enzyme activity. 3 Results PCF can reduce the mortality and apoptosis rate of UV-damaged Hela cells, and increase the intracellular Ca2+ content and succinate deoxygenase activity. PCF can also reduce the content of MDA and increase the activities of antioxidant enzymes GSH px, SOD and CAT. 4 Conclusions PCF may exert its protective effect on UV-damaged Hela cells by increasing the activity of antioxidant enzymes.