论文部分内容阅读
以聚苯乙烯胶乳为载体，制成乙型肝炎ｅ系统胶乳诊断试剂．以反向被动乳凝试验（ＲＰＬＡ）和乳凝抑制试验（ＬＡＩＴ）检测ＨＢｅＡｇ及抗－ＨＢｅ，并和ＥＬＩＳＡ法进行比较，结果发现受检５０份ＨＢｅＡｇ阳性血清及４１份抗－ＨＢｅ阳性标本的符合率为９６．０％和９１．１％，经统计学ｘ２检验无显著性差异．并且发现乳凝试验对乙型肝炎病毒表面和核心系统无交叉反应，对５份类风湿因子阳性血清和５０份健康人血清检测均为阴性，表明该法有良好的特异性（９８．１％）．此外本法与ＥＬＩＳＡ法相比，前者更具有操作简单，不需任何仪器设备，３～５ｍｉｎ出结果的特点，是检测乙型肝炎ｅ系统较为理想的方法之一，有推广应用价值．
Polystyrene latex as a carrier, made of hepatitis b system latex diagnostic reagent. HBeAg and anti-HBe were detected by reverse passive breast coagulation test (RPLA) and lacto-coagulation inhibition test (LAIT), and compared with the ELISA method, 50 HBeAg-positive sera and 41 anti-HBe positive specimens The coincidence rate was 96.0% and 91.1%, no significant difference by statistical x2 test. The results showed that there was no cross-reaction between the surface of hepatitis B virus and the core of the system, and the results of serum test of 5 rheumatoid factor-positive sera and 50 healthy people were negative, indicating that this method has good specificity (98.1% ). In addition, compared with the ELISA method, the former is more simple, without any equipment, 3 ~ 5min out of the results of the characteristics of hepatitis B e system is one of the more ideal way to promote the use of value.