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我们将Υ-(全氟辛酰胺基)丙基三乙氧基硅烷,CF_3(CF_2)_6CONH(CH_2)Si(OC_2H_5)_3,与堆积硅珠(比表面积300m~2/g)键合,使其表面改性.根据元素分析计算出此填料上的有机层复盖量为5％,键合基为Υ-(全氟辛酰胺基)丙基一乙氧基硅烷,表面层浓度0.3μmol/m~2.它的色谱极性较硅胶小,对多羟基甾体及共轭烯酮类化合物有较好的分离作用.曾应用于筋骨草激素、蜕皮激素、孕甾烯酮、螺旋内酯、副肾皮质酮、黄体酮及其它甾族化合物的分析分离.
We bonded γ- (perfluorooctamido) propyltriethoxysilane and CF_3 (CF_2) _6CONH (CH_2) Si (OC_2H_5) _3 to silica beads (specific surface area 300m ~ 2 / g) Its surface was modified.According to the elemental analysis, the coverage of organic layer on this filler was calculated to be 5%, the bonding group was Y- (perfluorooctanoamido) propyl monopropoxy silane, the surface layer concentration was 0.3μmol / m ~ 2. Its chromatographic polarity is smaller than that of silica gel, and has good separation effect on polyhydroxyl steroid and conjugated enone compounds. It has been applied to bungein, ecdysone, pregnenone, , Corticosterone, progesterone and other steroids.