羟脯氨酸(Hydroxyproline,HYP)作为衡量机体胶原代谢状况的一项指标,已在一些临床中得到应用。关于血尿羟脯氨酸的比色测定法,以往资料已有报导,这些报导的方法虽各有差异,但基本原理相同,即用酸将含有羟脯氨酸的多肽水解,然后用氯胺-T将羟脯氨酸氧化成吡咯,再与对二甲氨基苯甲醛(P-DMAB)反应,生成红色化合物,在560nm波长处比色测定。本文根据Ga-bor Huszar提出的碱水解法,对NaOH水解血清样品,比色测定血羟脯氨酸的方法 Hydroxyproline (HYP) as an indicator of the body’s metabolic status of collagen has been applied in some clinical trials. For hematuria hydroxyproline colorimetric assay, past data have been reported, although these methods vary, but the basic principle is the same, that is, the acid will hydroxyproline containing polypeptide hydrolysis, and then use chloramine - T oxidizes hydroxyproline to pyrrole and then reacts with p-dimethylaminobenzaldehyde (P-DMAB) to produce a red compound which is colorimetrically measured at a wavelength of 560 nm. According to the alkali hydrolysis method proposed by Ga-bor Huszar, the method of hydrolyzing serum sample by NaOH and measuring hydroxyproline by colorimetric method