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多发性骨髓瘤(MM)是一种B细胞恶变性疾病,特征表现为骨髓及周围血中出现大量恶变的浆细胞,即为浆细胞性白血病。近年发现骨髓瘤患者Tu/T_4和Tr/T_8细胞亚群不平衡,在缓解期常伴有T_8细胞绝对增多。某些资料提出,T细胞即通过白细胞间介素-2(IL-2)等可溶性因子对MM免疫调节方面起着重要作用。作者研究了29例MM患者IL-2的生成。其中8例在诊断期间,10例在完全缓解期(骨髓瘤细胞减少>75%),该10例至少已6个月未进行治疗。另11例在复发期。29例中16例(56%)为IgG骨髓瘤,10例(34%)为IgA骨髓瘤。3例(10%)为本周氏骨髓瘤。15例健康者作为对照组。应用Ficoll metnzoate融合法,在骨髓瘤病人和正常对照组离心分离出外周血单核细胞(PBMC),用AET处理绵羊红细胞,通过形成E-玫瑰花形能力鉴定T细胞。应用T_3、T_4及T_8单克隆抗体测定T细胞亚群。
Multiple myeloma (MM) is a degenerative disease of B cells characterized by a large number of malignant plasma cells in the bone marrow and peripheral blood, namely plasma cell leukemia. In recent years, myeloma found in patients with Tu / T_4 and Tr / T_8 cell subsets imbalance, often accompanied by remission T_8 cells absolute increase. Some sources suggest that T cells play an important role in immune regulation of MM through soluble factors such as interleukin-2 (IL-2). The authors studied IL-2 production in 29 MM patients. Of these, 8 were diagnosed and 10 were in complete remission (> 75% reduction in myeloma) and none of these 10 had been treated for at least 6 months. Another 11 cases in the recurrence. Sixteen (56%) of the 29 patients were IgG myeloma and 10 (34%) were IgA myeloma. Three cases (10%) of this week’s myeloma. Fifteen healthy people served as control group. Peripheral blood mononuclear cells (PBMCs) were isolated from myeloma patients and normal controls using Ficoll metnzoate fusion, sheep red blood cells were treated with AET, and T cells were identified by the ability to form an E-rosette. T cell subsets were determined by T_3, T_4 and T_8 monoclonal antibodies.