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目的：观察粉防己碱（Ｔｅｔ）对ＶＳＭＣ增殖的作用及对热应激蛋白７０ｋｄ（ＨＳＰ７０）及其ｍＲＮＡ和抑癌基因ｐ５３ｍＲＮＡ的影响。方法：用内皮素建立培养的血管平滑肌细胞增殖模型。采用氚－胸腺嘧啶核苷（［３Ｈ］ＴｄＲ）掺入法流式细胞术，Ｗｅｓｔｅｒｎ及Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交方法。结果：Ｔｅｔ能逆转内皮素所致的［３Ｈ］ＴｄＲ掺入量增多（Ｐ＜０．０１），阻止血管平滑肌细胞由静止期（Ｇ０／Ｇ１期）进入ＤＮＡ合成期（Ｓ期）和有丝分裂期（Ｇ２／Ｍ期），并能逆转内皮素引起的ＨＳＰ７０及ｍＲＮＡ表达增强（Ｐ＜０．０１或Ｐ＜０．０５），ｐ５３抑癌基因ｍＲＮＡ表达减弱（Ｐ＜０．０５）。结论：Ｔｅｔ能抑制血管平滑肌细胞增殖，与ＨＳＰ７０及ｐ５３的调控有关
Objective: To observe the effect of tetrandrine (Tet) on the proliferation of VSMC and its effect on 70kd (HSP70) and its mRNA and p53 mRNA. METHODS: Endothelins were used to establish cultured vascular smooth muscle cell proliferation models. Incorporation of thymidine-thymidine ([3H]TdR) by flow cytometry, Western and Northern blot hybridization methods. RESULTS: Tet could reverse [3H]TdR incorporation induced by endothelin (P<0.01), blocking vascular smooth muscle cells from quiescent phase (G0/G1 phase) into DNA synthesis phase (S phase) and mitosis (G2/M phase), and can reverse the expression of HSP70 and mRNA induced by endothelin (P<0.01 or P<0.05), and the expression of p53 tumor suppressor gene mRNA was weakened (P<0.05). Conclusion: Tet can inhibit the proliferation of vascular smooth muscle cells and is related to the regulation of HSP70 and p53