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目的探讨AGTExon3与食管癌高易感性之间的关系。方法聚合酶链反应(PCR)法,PCR基础上的限制性片段多态(PCR-RFLP)检测法及单链构象多态性分析(PCR-SSCP)检测法检测275例食管癌患者血标本抽提DNA,以正常血标本抽提DNA315例为对照。结果AGT第三外显子(AGTExon3)突变型携带者患ESCC的危险度比野生型携带者升高4.876倍(95%CI,3.248￣7.319)。将AGTExon3各基因型组合考查河南安阳地区人群患ESCC的结合危险度。在ESCC组,AGTExon30R值为0.1166,性别OR值为93.558,年龄为1.0703,具有统计学显著性意义。结论AGTExon3突变型基因型显著增加河南安阳地区人群ESCC的易感性,而DNA损伤修复酶基因缺陷可能是导致食管癌变的重要途径。
Objective To investigate the relationship between AGTExon3 and high susceptibility to esophageal cancer. Methods Polymerase chain reaction (PCR), restriction fragment length polymorphism (PCR-RFLP) based on PCR and single strand conformation polymorphism (PCR-SSCP) were used to detect blood samples from 275 patients with esophageal cancer To mention DNA, normal blood samples were extracted DNA315 cases as a control. Results AGT exon 3 (AGTExon3) mutant carriers had a 4.876-fold (95% CI, 3.248 to 7.319) risk of developing ESCC compared with wild-type carriers. The combination of AGTExon3 genotypes was used to investigate the combined risk of ESCC in Anyang area, Henan Province. In the ESCC group, the AGTExon30R value was 0.1166, the sex OR value was 93.558 and the age was 1.0703, with statistically significant significance. Conclusion AGTExon3 mutant genotypes significantly increase the susceptibility of ESCC in Anyang area, Henan Province. DNA damage repair gene defect may be an important pathway leading to esophageal cancer.