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目的:明确解毒化瘀颗粒对急性肝衰竭大鼠肝细胞有丝分裂指数和增殖指数的影响,为深入研究奠定基础。方法:以D-氨基半乳糖(D-Gal N)联合内毒素脂多糖(LPS)构建急性肝衰竭大鼠模型,在造模后6 h、24 h、48 h每组抽取6只大鼠采取标本,进行肝细胞有丝分裂指数和增殖指数检测。结果:急性肝衰竭大鼠经解毒化瘀颗粒干预治疗后,肝细胞在造模后24 h、48 h有丝分裂指数和增殖指数明显提高,与模型对照组比较差异具有统计学意义(P<0.05)。结论:解毒化瘀颗粒可促进急性肝衰竭大鼠肝细胞增殖与分裂,其机制与提高肝细胞在S期DNA复制有关。
Objective: To clarify the effect of Jiedu Huayu Granule on mitosis index and proliferation index of hepatocytes in rats with acute hepatic failure, and lay the foundation for further study. Methods: A rat model of acute liver failure was established by D-Gal N combined with lipopolysaccharide (LPS). Six rats in each group were taken at 6 h, 24 h and 48 h after model establishment Specimens were tested for hepatocyte mitosis index and proliferation index. Results: After treatment with Jiedu Huayu granule, the mitotic index and proliferation index of hepatocytes increased significantly at 24 h and 48 h after modeling, showing significant difference from the model control group (P <0.05) . Conclusion: Jiedu Huayu granules can promote the proliferation and division of hepatocytes in rats with acute liver failure. The mechanism is related to the enhancement of DNA replication in hepatocytes in S phase.