目的:探讨女性乳腺癌患者人乳头状瘤病毒(HPV16、HPV18)检测的临床价值。方法:选择我国西北地区经病理检查确诊的乳腺癌80例为观察组,乳腺良性疾病80例为对照组,采用实时荧光定量PCR技术(FQ-PCR)及透射电镜技术检测两组病例组织中HPV16、HPV18感染情况。结果:采用FQ-PCR及透射电镜技术两组病变组织中均未检测出HPV16、HPV18。结论:在我国西北地区,HPV16、HPV18与女性乳腺癌的发生、发展并无直接确切的关系。 Objective: To investigate the clinical value of detecting human papillomavirus (HPV16, HPV18) in female breast cancer patients. Methods: 80 cases of breast cancer diagnosed by pathology in Northwest China were selected as observation group and 80 cases of benign breast disease as control group. Real-time fluorescence quantitative PCR (FQ-PCR) and transmission electron microscopy were used to detect the expression of HPV16 , HPV18 infection. Results: HPV16 and HPV18 were not detected in the two groups by FQ-PCR and transmission electron microscopy. Conclusion: In the northwestern China, HPV16 and HPV18 have no direct and definite relationship with the occurrence and development of female breast cancer.