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为了解白细胞介素６（ＩＬ－６）基因转染的高分泌ＩＬ－６的ＦＢＬ－３红白血病细胞（ＦＢＬ－３－ＩＬ－６＋）株体内外的生物学特性，动态观察在不同剂量６０Ｃｏγ射线照射后其ＩＬ－６的分泌水平，同时动态观察经腹腔、局部皮下注射途径将其移植入Ｃ５７ＢＬ／６小鼠体内后，血清和局部细胞培养上清液中ＩＬ－６含量。结果表明，ＦＢＬ－３－ＩＬ－６＋细胞以８Ｇｙ照射剂量为制备瘤苗的最佳照射剂量，照射后２周内一直保持较高水平的ＩＬ－６分泌量。体内移植时，经腹腔途径注入后可测得一定浓度的血清ＩＬ－６，皮下注入时注射局部细胞培养上清中亦有一定水平的ＩＬ－６。为将该细胞作为治疗白血病瘤苗及其注射间隔时间提供了实验依据。
To investigate the biological characteristics of FBL-3 erythroleukemia cells (FBL-3-IL-6 +) secreting IL-6 secreted by interleukin 6 (IL-6) gene in vitro and in vivo, The levels of IL-6 in serum and local cell culture supernatants were transplanted into C57BL / 6 mice by intraperitoneal and local subcutaneous injection. The results showed that FBL-3-IL-6 + cells at 8Gy dose for the preparation of tumor vaccine optimal dose, 2 weeks after irradiation has been maintained at a high level of IL-6 secretion. When transplanted in vivo, a certain concentration of serum IL-6 can be detected after intraperitoneal injection, and there is also a certain level of IL-6 in the supernatant of the local cell culture injected subcutaneously. To provide an experimental basis for treating this cell as a leukemia vaccine and its injection interval.