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本研究采用体外细胞培养分组对比法检测肝再生刺激因子(HSS)、胰岛素或/和胰高血糖素对大鼠原代培养肝细胞和人肝肿瘤细胞株HepG_2 DNA合成(肝细胞再生)的作用。结果发现,HSS无论对大鼠肝细胞或人肝肿瘤细胞均具有较强的刺激DNA合成的作用(与对照组相比分别增加1.14和1.05倍,P<0.01)。而对人纤维母细胞则无促进DNA合成的作用。低浓度胰岛素或/和胰高血糖素对肝细胞DNA合成无作用。高浓度胰岛素(10~(-5)M)具有中等度促进肝再生的作用(与对照组相比增加40％,P<0.05)。两者联合应用(G+I)似无协同作用。据此,我们认为,HSS是一种潜在的促进肝再生的有效因素,可能具有临床实用价值。体外应用优于现在临床广泛应用的胰岛素加胰高血糖素(G+I)疗法。
In this study, the effect of hepatic regeneration stimulator (HSS), insulin and / or glucagon on DNA synthesis of primary cultured hepatocytes and human hepatoma HepG2 cells (hepatocyte regeneration) was detected by cell culture in vitro. . The results showed that HSS had a strong stimulating effect on DNA synthesis in both rat hepatocytes and human hepatoma cells (1.14 and 1.05 folds, respectively, compared with the control group, P <0.01). The human fibroblasts did not promote the role of DNA synthesis. Low concentrations of insulin and / or glucagon have no effect on hepatocyte DNA synthesis. High concentrations of insulin (10-5 M) had a moderate effect on liver regeneration (40% increase over control, P <0.05). The combination of the two (G + I) seems to have no synergistic effect. Accordingly, we believe that HSS is a potential effective factor to promote liver regeneration, which may have clinical value. In vitro application is superior to the currently widely used clinical insulin plus glucagon (G + I) therapy.