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目的：研究喂养边缘缺乏水平和充裕水平钙、铁、锌含量的饲料对大鼠生长发育，营养状况及生殖机能的影响，并探讨三种营养素间的相互作用。方法：采用三因素两水平的正交设计，将四周龄Ｗｉｓｔａｒ雌性大鼠按体重随机分成８组，分别饲以不同钙、铁、锌含量的饲料，１６周后与正常雄鼠合笼交配。以发现阴栓日计为妊娠第０天，妊娠第２０天时处死孕鼠并剥离胎盘和胎鼠，测定各项指标。结果：饲料中不同钙、铁、锌水平对大鼠营养状况有显著影响。大鼠血清铁水平分别受饲料钙、铁水平独立作用的影响，血清锌水平分别受饲料铁、锌水平独立作用的影响，饲料中铁、锌水平及钙与铁的交互作用对大鼠血清碱性磷酸酶的活性有显著影响，红细胞游离原卟啉（ＦＥＰ）含量受饲料铁水平及钙与铁交互作用的影响，饲料中不同钙、铁、锌水平对大鼠血红蛋白含量无显著影响。饲料中不同钙、铁、锌水平对母鼠生殖机能未见显著影响。结论：边缘缺乏水平的钙、铁、锌对大鼠的生殖机能无显著影响，但对大鼠营养状况有直接影响，导致体内营养素储备降低，尤其是处于边缘缺乏水平的铁和锌更容易受到饲料中其它元素的影响而加剧营养缺乏。
OBJECTIVE: To study the effects of feed with insufficient marginal levels and adequate levels of calcium, iron and zinc on the growth, nutritional status and reproductive function of rats and to explore the interaction between the three nutrients. METHODS: Female Wistar rats of 4 weeks old were randomly divided into 8 groups according to the three factors and two levels of orthogonal design. The rats were fed with different dietary calcium, iron and zinc respectively. After 16 weeks, they were mated with normal male mice. In order to find that the day of vaginal suppository was 0 day of pregnancy, the pregnant rats were sacrificed on the 20th day of gestation and the placenta and fetus were dissected, and the indexes were measured. Results: Different dietary calcium, iron and zinc levels had significant effects on the nutritional status of rats. Serum iron levels in rats were independently influenced by dietary calcium and iron levels. Serum zinc levels were independently influenced by dietary iron and zinc levels. Iron and zinc levels in diet and the interaction between calcium and iron on serum alkaline The activity of phosphatase was significantly affected. The content of erythrocyte free protoporphyrin (FEP) was influenced by the level of feed iron and the interaction between calcium and iron. The dietary calcium, iron and zinc levels had no significant effect on the hemoglobin content of rats. Feeds of different calcium, iron, zinc levels on the reproductive performance of female rats had no significant effect. CONCLUSION: Lack of calcium, iron and zinc at the marginal edge have no significant effect on the reproductive function of rats, but have a direct effect on the nutritional status of rats, leading to the reduction of nutrient reserves in the body. In particular, iron and zinc in marginal deficiencies are more susceptible The impact of other elements in feed exacerbates nutritional deficiencies.