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Gene libraries of Rana nigromaculata and Mus musculus (C_(57)J_6) have been constructed bythe cosmid system developed by Collins using BamHl and Pstl as cloning restriction enzymes.A simplified formula, which is a modification of Clarke and Carbon derived from apprixima-tion, was used for calculating the number of colonies or recombinants necessary for theconstruction of a gene library. Pro~+ and leu~+ complementary to the recipient cells (RB101)with the genetic constitution of pro~- and leu~- have been selected from the BamHl genelibraries of both Rana nigromaculata and the mouse in the present work.
Gene libraries of Rana nigromaculata and Mus musculus (C_ (57) J_6) have been constructed by the cosmid system developed by Collins using BamHl and Pstl as cloning restriction enzymes. A simplified formula, which is a modification of Clarke and Carbon derived from apprixima tion , was used for calculating the number of colonies or recombinants necessary for the construction of a gene library. Pro ~ + and leu ~ + complementary to the recipient cells (RB101) with the genetic constitution of pro ~ - and leu ~ - have been selected from the BamHl genelibraries of both Rana nigromaculata and the mouse in the present work.