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目的 :通过观察局灶性脑缺血 /再灌注后大鼠脑组织中 Fas、肿瘤坏死因子 - α( TNF- α)的动态变化及细胞凋亡的情况 ,探讨脑缺血后 Fas、肿瘤坏死因子 - α与细胞凋亡的关系。方法 :采用大鼠大脑中动脉线栓动物模型 ,应用免疫组化及 TUNEL方法检测 Fas、TNF- α的动态变化和细胞凋亡情况并进行图像分析。结果 :局灶性脑缺血再灌注后 Fas过度表达 ,且在缺血再灌注后 6h达高峰 ,于 2 4 h开始下降 ;TNF- α的表达于再灌注 3h已有明显升高 ,2 4 h达到高峰 ,其后逐渐下降 ;细胞凋亡于再灌注 6h开始增加 ,2 4 h达到高峰 ,36h略有下降 ,且与 Fas及 TNF- α表达范围基本一致。结论 :Fas、TNF- α在局灶性脑缺血 /再灌注损伤中表达增加 ,介导细胞凋亡。它们在局灶性脑缺血再灌注损伤的病理过程中起到重要作用。
OBJECTIVE: To investigate the dynamic changes of Fas and TNF-α in rat brain after focal cerebral ischemia / reperfusion and the effects of apoptosis on the expression of Fas, tumor necrosis Relationship between factor - α and apoptosis. Methods: Rat middle cerebral artery occlusion model was established. The dynamic changes of Fas, TNF-α and apoptosis were detected by immunohistochemistry and TUNEL method and analyzed by image analysis. Results: Fas was over-expressed after focal cerebral ischemia-reperfusion and peaked at 6h after ischemia-reperfusion and began to decline at 24 h. The expression of TNF-α was significantly increased at 3 h after reperfusion h peaked and then decreased gradually. Apoptosis began to increase at 6h after reperfusion, peaked at 24 h and decreased slightly at 36 h, which was consistent with the expression of Fas and TNF-α. Conclusion: The expressions of Fas and TNF-α are increased in focal cerebral ischemia / reperfusion injury and mediate apoptosis. They play an important role in the pathological process of focal cerebral ischemia-reperfusion injury.