论文部分内容阅读
作者通过对获自世界各地甲型肝炎流行区的HAV分离株的核酸序列测定,分析了HAV野毒株的基因型,并研究了遗传变异情况.测定方法采用PCR技术,加入正、反意义引物,通过逆转录在野生型HAV的VP1基因组区段内扩增出一个247bp片段.从22个不同流行区获得的HAV分离株的序列可分为3个遗传组描述.对同一次甲型肝炎爆发中的分离株仅选一种,而且不包括由黑猩猩传代的分离株.作者共鉴定出3个不同的分支,彼此间相差约20%、10%和5%.一般来说,从同
The authors analyzed the genotypes of wild-type HAV isolates from HAV isolates from endemic areas of Hepatitis A worldwide and investigated their genetic variation.Methods of PCR Assay was performed using positive and negative sense primers , A 247 bp fragment was amplified by reverse transcription in the VP1 genome segment of wild-type HAV.The sequences of HAV isolates obtained from 22 different endemic areas can be divided into three genetic groups Description For the same hepatitis A outbreak Of the isolates selected only one, and does not include the passage of chimpanzee isolates identified a total of three different branches, with a difference of about 20%, 10% and 5%. In general, from the same